Publications
2023
Yang J, Lee Y, Hwang CS. The ubiquitin-proteasome system links NADPH metabolism to ferroptosis. Trends in Cell Biology. 2023 Aug 7:S0962-8924(23)00136-8. doi: 10.1016/j.tcb.2023.07.003.
Mun SH, Lee CS, Kim HJ, Kim J, Lee H, Yang J, Im SH, Kim JH, Seong JK, Hwang CS.Marchf6 E3 ubiquitin ligase critically regulates endoplasmic reticulum stress, ferroptosis, and metabolic homeostasis in POMC neurons. Cell Reports. 2023 Jul 7;42(7):112746.
Kim D, Seok OH, Ju S, Kim SY, Kim JM, Lee C, Hwang CS. Detection of Nα-terminally formylated native proteins by a pan-N-formyl methionine-specific antibody. J Biol Chem. 2023 Mar 27:104652. doi: 10.1016/j.jbc.2023.104652.
Seo DY, Kim D, Nguyen KT, Oh J, Lee JS, Hwang CS. N-Terminally arginylated ubiquitin is attached to histone H2A by RING1B E3 ligase in human cells. Biochem Biophys Res Commun. 2023 Feb 10:S0006-291X(23)00194-8.
2022
Nguyen KT, Mun SH, Yang J, Lee J, Seok OH, Kim E, Kim D, An SY, Seo DY, Suh JY, Lee Y, Hwang CS. The MARCHF6 E3 ubiquitin ligase acts as an NADPH sensor for the regulation of ferroptosis. Nat Cell Biol. 2022 Aug;24(8):1239-1251. doi: 10.1038/s41556-022-00973-1. Epub 2022 Aug 8.PMID: 35941365
Nguyen KT, Ju S, Kim SY, Lee CS, Lee C, Hwang CS. N-Terminal Modifications of Ubiquitin via Methionine Excision, Deamination, and Arginylation Expand the Ubiquitin Code. Mol Cells. 2022 Mar 31;45(3):158-167. doi: 10.14348/molcells.2022.2027. PMID: 35253.
Lee CS, Kim D, Hwang CS. Where Does N-Formylmethionine Come from? What for? Where Is It Going? What is the origin of N-formylmethionine in eukaryotic cells? Mol Cells. 2022 Mar 31;45(3):109-111. doi: 10.14348/molcells.2021.5040. PMID: 35288488.
Kim BH, Kim MK, Oh SJ, Nguyen KT, Kim JH, Varshavsky A, Hwang CS, Song HK. Crystal structure of the Ate1 arginyl-tRNA-protein transferase and arginylation of N-degron substrates. Proc Natl Acad Sci U S A. 2022 Aug 2;119(31):e2209597119. doi: 10.1073.
2020
Seo JH, Hwang CS, Yoo JY. MTFMT deficiency correlates with reduced mitochondrial integrity and enhanced host susceptibility to intracellular infection. Sci Rep. 2020 Jul 7;10(1):11183.
Ju S, Kwon Y, Kim JM, Park D, Lee S, Lee JW, Hwang CS, Lee C. iNrich, Rapid and Robust Method to Enrich N-Terminal Proteome in a Highly Multiplexed Platform, Anal Chem. 2020 May 5;92(9):6462-6469.
Lee DW, Lee S, Min CK, Park C, Kim JM, Hwang CS, Park SK, Cho NH, Hwang I. Cross-Species Functional Conservation and Possible Origin of the N-Terminal Specificity Domain of Mitochondrial Presequences. Front Plant Sci. 2020 Feb 13;11:64.
2019
Nguyen KT, Kim JM, Park SE, Hwang CS. N-terminal methionine excision of proteins creates tertiary destabilizing N-degrons of the Arg/N-end rule pathway. J Biol Chem. 2019 Mar 22;294(12):4464-4476. doi: 10.1074/jbc.RA118.006913. Epub 2019 Jan 23. PMID: 30674553.
Nguyen KT, Lee CS, Mun SH, Truong NT, Park SK, Hwang CS. N-terminal acetylation and the N-end rule pathway control degradation of the lipid droplet protein PLIN2. J Biol Chem. 2019 Jan 4;294(1):379-388. doi: 10.1074/jbc.RA118.005556. Epub 2018 Nov 13. PMID: 30425097.
2018
Kim JM, Seok OH, Ju S, Heo JE, Yeom J, Kim DS, Yoo JY, Varshavsky A, Lee C, Hwang CS. Formyl-methionine as an N-degron of a eukaryotic N-end rule pathway. Science. 2018 Nov 30;362(6418):eaat0174. doi: 10.1126/science.aat0174. Epub 2018 Nov 8. PMID: 30409808.
Nguyen KT, Mun SH, Lee CS, Hwang CS. Control of protein degradation by N-terminal acetylation and the N-end rule pathway. Exp Mol Med. 2018 Jul 27;50(7):1-8. doi: 10.1038/s12276-018-0097-y. PMID: 30054456.
2016
Lee KE, Heo JE, Kim JM, Hwang CS. N-Terminal Acetylation-Targeted N-End Rule Proteolytic System: The Ac/N-End Rule Pathway. 2016 Mar;39(3):169-78. doi: 10.14348/molcells.2016.2329. Epub 2016 Feb 16. PMID: 26883906.
Wadas B, Borjigin J, Huang Z, Oh JH, Hwang CS, Varshavsky A. Degradation of Serotonin N-Acetyltransferase, a Circadian Regulator, by the N-end Rule Pathway. J Biol Chem. 2016 Aug 12;291(33):17178-96. doi: 10.1074/jbc.M116.734640. Epub 2016 Jun 23. PMID: 27339900.
Park MJ, Sheng R, Silkov A, Jung DJ, Wang ZG, Xin Y, Kim H, Thiagarajan-Rosenkranz P, Song S, Yoon Y, Nam W, Kim I, Kim E, Lee DG, Chen Y, Singaram I, Wang L, Jang MH, Hwang CS, Honig B, Ryu S, Lorieau J, Kim YM, Cho W. SH2 Domains Serve as Lipid-Binding Modules for pTyr-Signaling Proteins. Mol Cell. 2016 Apr 7;62(1):7-20.
2015
Park SE, Kim JM, Seok OH, Cho H, Wadas B, Kim SY, Varshavsky A, Hwang CS. Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Science. 2015 Mar 13;347(6227):1249-1252. doi: 10.1126/science.aaa3844. PMID: 25766235.
Piatkov K, Vu TTM, Hwang CS, Varshavsky A Formyl-methionine as a degradation signal at the N-termini of bacterial proteins. Microb Cell. 2015;2(10):376-393. doi: 10.15698/mic2015.10.231. PMID: 26866044.
Kim J, Saint-Andre C, Lim H, Hwang CS, Egly JM, Cho Y (2015) Crystal Structure of the Rad3/XPD regulatory domain of Ssl1/p44. J Biol Chem 290(13):8321-8330.
Sun HY, Kim N, Hwang CS, Yoo JY. Protein Degradation of RNA Polymerase II-Association Factor 1(PAF1) Is Controlled by CNOT4 and 26S Proteasome. PLoS One. 2015 May 1;10(5):e0125599.
2014
Kim HK, Kim RR, Oh JH, Cho H, Varshavsky A, Hwang CS. The N-terminal methionine of cellular proteins as a degradation signal. Cell. 2014 Jan 16;156(1-2):158-69. doi: 10.1016/j.cell.2013.11.031. Epub 2013 Dec 19. PMID: 24361105.
Kim JM and Hwang CS (2014) Crosstalk between the Arg/N-end and Ac/N-end rule. Cell Cycle. 2014;13(9):1366-7. doi: 10.4161/cc.28751. Epub 2014 Apr 3. PMID: 24698805.
Lee KE#, Ahn JY#, Kim JM and Hwang CS Synthetic lethal screen of NAA20, a catalytic subunit gene of NatB N-terminal acetylase in Saccharomyces cerevisiae. J Microbiol. 2014 Oct;52(10):842-8. doi: 10.1007/s12275-014-3694-z. Epub 2014 Aug 27. PMID: 25163837.
2013
Shemorry A, Hwang CS, Varshavsky A. Control of protein quality and stoichiometries by N-terminal acetylation and the N-end rule pathway. Mol Cell. 2013 May 23;50(4):540-51. doi: 10.1016/j.molcel.2013.03.018. Epub 2013 Apr 18. PMID: 23603116.
2011
Hwang CS, Sukalo M, Batygin O, Addor MC, Brunner H, Aytes AP, Mayerle J, Song HK, Varshavsky A, Zenker M. Ubiquitin ligases of the N-end rule pathway: assessment of mutations in UBR1 that cause the Johanson-Blizzard syndrome. PLoS One. 2011;6(9):e24925. doi: 10.1371/journal.pone.0024925. Epub 2011 Sep 13. PMID: 21931868.
2010
Hwang CS, Shemorry A, Varshavsky A. N-terminal acetylation of cellular proteins creates specific degradation signals. Science. 2010 Feb 19;327(5968):973-7. doi: 10.1126/science.1183147. Epub 2010 Jan 28.PMID: 20110468.
Hwang CS, Shemorry A, Auerbach D, Varshavsky A. The N-end rule pathway is mediated by a complex of the RING-type Ubr1 and HECT-type Ufd4 ubiquitin ligases. Nat Cell Biol. 2010 Dec;12(12):1177-85. doi: 10.1038/ncb2121. Epub 2010 Nov 14.
Hwang CS, Shemorry A, Auerbach D, Varshavsky A. Ubiquitin ligases of the N-end rule pathway: assessment of mutations in UBR1 that cause the Johanson-Blizzard syndrome. PLoS One. 2011;6(9):e24925. doi: 10.1371/journal.pone.0024925. Epub 2011 Sep 13 .PMID: 21931868.
2009
Hwang CS, Shemorry A and Varshavsky A (2009) Two proteolytic pathways regulate DNA repair by co-targeting the Mgt1 alkylguanine transferase. Proc Natl Acad Sci USA 109, 2142-2147.
2008
Hwang CS, Varshavsky A. Regulation of peptide import through phosphorylation of Ubr1, the ubiquitin ligase of the N-end rule pathway. Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19188-93.
Xia Z, Turner GC, Hwang CS, Byrd C, Varshavsky A. Amino acids induce peptide uptake via accelerated degradation of CUP9, the transcriptional repressor of the PTR2 peptide transporter. J Biol Chem. 2008 Oct 24;283(43):28958-68.
2003
Hwang CS, Baek YU, Yim HS and Kang, SO (2003) Protective roles of mitochondrial manganese-containing superoxide dismutase against various stresses from Candida albicans. Yeast 20, 929-941.
Hwang CS, Oh JH, Huh WK, Yim HS and Kang SO (2003) Ssn6, an important factor of morphological conversion and virulence in Candida albicans. Mol Microbiol 47, 1029-1043. (Cover section).
2002
Hwang CS, Rhie Ge, Oh JH, Huh WK, Yim HS and Kang, SO (2002) Copper- and zinc-containing superoxide dismutase is required for the protection from oxidative stresses and full virulence in Candida albicans. Microbiology 148, 3705-3713.
2001
Lee JS, Huh WK, Lee BH, Baek, YU, Hwang CS, Kim ST, Kim, YR and Kang SO (2001) Mitochondrial NADH-cytochrome b(5) reductase plays a crucial role in the reduction of D-erythroascorbyl free radical in Saccharomyces cerevisiae. Biochim Biophys Acta 1527, 31-38.
1999
Cho SH, Na JU, Youn H, Hwang CS, Lee CH and Kang SO (1999) Sepiapterin reductase producing L-threo-dihydrobiopterin from Chlorobium tepidum. Biochem J 340, 497-503.
Hwang CS, Rhie Ge, Kim ST, Kim YR, Huh WK, Baek YU and Kang SO (1999) Copper-and zinc-containing superoxide dismutase and its gene from Candida albicans. Biochim Biophys Acta 1427, 245-255.
Rhie, Ge, Hwang CS, Brady MJ, Kim ST, Kim YR, Huh WK, Baek YU, Lee BH, Lee JS and Kang SO (1999) Manganese-containing superoxide dismutase and its gene from Candida albicans. Biochim Biophys Acta 1426, 409-419.
1998
Huh WK, Lee BH, Kim ST, Kim YR, Rhie Ge, Baek YW, Hwang CS, Lee JS and Kang SO (1998) D-Erythroascorbic acid is an important antioxidant molecule in Saccharomyces cerevisiae. Mol Microbiol 30, 95-903.
Cho SH, Na JU, Youn H, Hwang CS, Lee CH and Kang, SO (1998) Tepidopterin, threo-biopterin beta-N-acetylglucosamine from Chlorobium tepidum. Biochim Biophys Acta 1379, 53-60.
